The energy consumption of the data center cooling system accounts for 40% of the total energy consumption. Reasonable air flow organization can effectively improve the cooling efficiency of the data center and improve the high energy consumption of the data center. In order to optimize air organization of data center, the impact of air supply methods, cold aisle containment, and return air vent positions on the thermal environment are first qualitatively analyzed according to the numerical simulation results. Secondly, the non-uniformity coefficient is introducedto quantitatively investigate the effects of air conditioning layout, perforated raised floor porosity, and raised floor height on air flow uniformity using the orthogonal test method. Based on a case study of a data center in East China, the conclusion is that using raised floor air supply and return air, fully enclosing the cold aisle, and placing the return air outlet above the hot aisle can improve the thermal environment performance of the data center.