Regional integrated energy system improves the economy and environment of energy supply viacollaborative optimal operation of power, gas, cold and heat energy systems. However, the coupling of multiple energy sources increases the complexity of system operation. Reasonable operation optimization method is the key point to ensure its economy. The structure of the regional integrated energy system that consists of multiple energy stations is analyzed. A practical model of the multi-energy station regional integrated energy system is established. On this basis, a collaborative optimal dispatching method for the regional integrated energy system with multiple energy stations is proposed, which aims at the economic operation of the system. The simulation results of a practical case show that collaborative optimal operation for multiple energy stations can significantly improve the overall economy of the regional integrated energy system.