In order to research the problem of line loss allocation in distribution network with distributed generators from the perspective of economic operation, a calculation method of line loss allocation based on economic operation analysis for distribution network with distributed generators is proposed. Firstly, the minimum line loss of distribution network is calculated by using equivalent resistance method, then the economic operation load interval of the distribution network before and after the distributed generator is connected is calculated. Secondly, the reasons of the economic operation load interval change before and after the distribution network is connected to the distributed generator are analyzed, and different operation scenarios are constructed accordingly. Finally, the line loss allocation of distributed generators is calculated by using the power flow tracing algorithm, and the calculated line loss allocation is corrected by setting the corresponding reward and punishment coefficients. The results of example analysis verify the validity and rationality of the proposed method.